Phospho-rps6

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August undefined, 2024

WebNational Center for Biotechnology Information WebProduct name Anti-RPS6 (phospho S235) antibody [EP1338 (2)Y] - Ribosome Marker See all RPS6 primary antibodies Description Rabbit monoclonal [EP1338 (2)Y] to RPS6 (phospho …

Purified anti-RPS6 Phospho (Ser235/Ser236) Antibody

WebImmunohistochemical analysis of Phospho-RPS6 pSer240 in paraffin-embedded human breast carcinoma using Phospho-RPS6 pSer240 polyclonal antibody (Product # PA5-36759) at a dilution of 1:100. Western blot analysis of Phospho-S6 (Ser240) in Lane 1: HeLa whole cell lysate (40 µg), Lane 2: HeLa treated with serum starved (24h) whole cell lysate (40 ... WebWestern blot analysis of Phospho-RPS6 (Ser240, Ser244) in untreated (–) and treated (+) 293T whole cell extract using Phospho-RPS6 (Ser240, Ser244) polyclonal antibody ( Product # PA5-85822) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. fish tank filter care tips

Phospho-S6 Ribosomal Protein (Ser235/236) Antibody

WebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also detected with immunoblotting. b and c HEK293T and SW480 cells were transfected with FLAG empty vector or FLAG-DRAM1 plasmids for 24 h, and the cells were starved for … WebAnimals were fasted overnight and refed ad lib for 4 h. Quantification of phospho-rpS6 is shown at the right, normalized to total rpS6. (*) P < 0.05 by Student's t-test. (D) … WebQuality Control: the Phospho-RPS6 (S240/S244) assay kit is routinely tested against Insulin-treated MCF7 lysates. MCF7 cells were cultured in a T175 flask to 90% confluence and stimulated with 10 µM of insulin for 60 min at RT. Following cell lysis using 16 mL of 1X Lysis Buffer 4, lysates candy bar in a jar thm

Phospho-Ribosomal Protein S6 (Ser235/236) AlphaLISA ... - PerkinElmer

Phospho-S6 (Ser240, Ser244) Recombinant Monoclonal Antibody …

WebFeb 6, 2024 · RPS6 is phosphorylated downstream of active EGF signaling 13 and is lost within the first 24 hours of AG1478 treatment (Supplementary Fig. 4A). Quantification of … WebRayBio® Phospho-RPS6 (S235) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated RPS6 protein in your experimental model system, you can verify pathway activation in your cell lysates. candy bar imagesWebJan 14, 2013 · S6 kinases (S6Ks) are mechanistic target of rapamycin substrates that participate in cell growth control. S6Ks phosphorylate ribosomal protein S6 (rpS6) and additional proteins involved in the ... candy bar images clip art

"WebNational Center for Biotechnology Information " - Phospho-rps6

Phospho-rps6

Regulation of Ribosomal Protein S6 Phosphorylation by Casein …

WebMar 11, 2011 · Carboxyl-terminal phosphorylation of rpS6 is regulated by at least two signal transduction pathways. The p70 ribosomal S6 kinases, S6K1 and S6K2, play a major role in rpS6 C terminus phosphorylation in response to insulin, serum, and amino acid stimulation ( … WebMar 9, 2024 · Anti-RPS6 (phospho S235 + S236) antibody [SP50] See all RPS6 primary antibodies Description Rabbit monoclonal [SP50] to RPS6 (phospho S235 + S236) Host species Rabbit Tested applications Suitable for: Dot blot, IHC-P, ICC/IF, Flow Cyt more details Species reactivity Reacts with: Mouse, Human Immunogen

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WebThe phosphorylation of ribosomal protein S6 (rpS6) has been described for the first time about four decades ago. Since then, numerous studies have shown that this modification … WebMar 9, 2024 · Levels of phosphorylated RPS6 (p-RPS6) and S6 kinases (p-S6K) are shown to indicate the effectiveness of rapamycin at inhibiting mTORC1 activity.

WebFeb 6, 2024 · We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis of the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully... WebRPS6 Rabbit Polyclonal Antibody: 50μl: 593.00元: 加入购物车: AG3129: Phospho-S6 Ribosomal Protein (Ser235/236) Mouse Monoclonal Antibody: 50μl: 998.00元: 加入购物车: AG3133: Phospho-S6 Ribosomal Protein (Ser235/Ser236) Rabbit Monoclonal Antibody: 50μl: 998.00元: 加入购物车: AG3136: Phospho-S6 Ribosomal Protein (Ser240/244 ...

WebRPS6 Antibodies Target Information Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. WebRPS6 is a 249 amino acid protein with a predicted molecular weight of 28.6 kD. Distribution Cytosol, nucleus, ER, ubiquitous expression. Function Phosphorylation events at a cluster …

WebApr 10, 2024 · Purpose: To present a case of a chemotherapy regimen combining a fibroblast growth factor receptor (FGFR) and mitogen-activated protein kinase kinase …

Web兔抗RPS6 (Phospho-Ser235)多克隆抗体由上海纪宁整理，包括兔抗RPS6 (Phospho-Ser235)多克隆抗体使用说明书、价格表、应用范围、使用注意事项及操作步骤等资讯。 candy bar hello kittyWebCells were then intracellularly stained with 5 µg/mL (1:100 dilution) of Purified anti-RPS6 Phospho (Ser244) Antibody overnight at 4°C, after which proteins were visualized with Alexa Fluor® 594 Goat anti-mouse IgG Antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective. candy bar inflationWebMay 3, 2024 · (i) Ribosomal protein S6 (rpS6) is a downstream substrate of PI3K/akt/mTOR/ kinase and MAPK kinase pathways and its dephosphorylation is also triggered by DNA double strand breaks. Phospho-rpS6 is reliably measurable by flow cytometry and thus has the potential to function as a biomarker of responsiveness to several therapeutic agents. candy bar in a silver wrapperWebRPS6 is a 249 amino acid protein with a predicted molecular weight of 28.6 kD. Distribution Cytosol, nucleus, ER, ubiquitous expression. Function Phosphorylation events at a cluster … candy bar in pool caddyshackWebAnimals were fasted overnight and refed ad lib for 4 h. Quantification of phospho-rpS6 is shown at the right, normalized to total rpS6. (*) P < 0.05 by Student's t-test. (D) Immunofluorescence staining of phospho-S6 (red) and TFE3 (green) in control (NTC) cells and cells lacking TSC1 or TSC2. Cells were starved or starved and restimulated with ... candy bar induction ceremonyWebMetformin lowers Ser-129 phosphorylated α-synuclein levels via mTOR-dependent protein phosphatase 2A activation fish tank filter dividersWebAll lanes : Anti-RPS6 (phospho S240 + S244) antibody [EPR20770] (ab215214) at 1/5000 dilution Lane 1 : MCF7 (human breast adenocarcinoma cell line) grown in serum-free media overnight, whole cell lysate Lane 2 : MCF7 grown in serum-free media overnight, then grown in 20% FBS media for 30 minutes, whole cell lysate Lane 3 : NIH/3T3 (mouse embryonic … candy bar in prison