Fastpfu polymerase
WebSep 18, 2024 · The formal PCR test used TransGen AP221-02: TransStart Fastpfu DNA Polymerase, 20 μl reaction system: 5×FastPfu buffer 4 μl, 2.5 mM dNTPs 2 μl, forward primer (5 μM) 0.8 μl, reverse primer (5 μM) 0.8 μl, FastPfu polymerase 0.4 μl, BSA 0.2 μl, template DNA 10 ng, and supplement ddH 2 O to 20 μl. The following thermal cycling … WebFeb 17, 2024 · The amplification system was 20ul, 4ul 5*FastPfu buffer, 2ul 2.5 mM dNTPs, 0.8ul primer (5uM), 0.4ul FastPfu polymerase; 10 ng DNA template. Purified amplicons were pooled in equimolar amounts and paired-end sequenced on an Illumina MiSeq PE300 platform/NovaSeq PE250 platform (Illumina, San Diego, USA) according to the standard …
Fastpfu polymerase
Did you know?
WebTransStart ® FastPfu DNA polymerase (FastPfu), TransStart ® FastPfu Fly DNA polymerase (FastPfu Fly) and EasyPfu DNA Polymerase (EasyPfu) were purchased from TransGen Biotech. Proteinase K from Tritirachium album limber and elastase from the porcine pancreas were purchased from Roche (Mannheim, Germany) and Sangon … WebPfu Polymerase is a thermostable DNA polymerase isolated from the archaeal thermophile Pyrococcus furiosus. The 90-kDa Pfu polymerase enzyme features a 3’-5’ exonuclease …
WebApr 14, 2024 · The polymerase in the PCR process for bacterial/fungal DNA was TransStart Fastpfu DNA Polymerase/TaKaRa rTaq DNA polymerase. Twenty-microliter bacteria/fungi mixtures for PCR contained 4 μL of 5× TransStart FastPfu buffer/2 μL of 10× Buffer, 2.5 mM dNTPs in 2 μL, 0.8 μL of forward primer (5 μM), 0.8 μL of reverse primer (5 μM), 0.4 μL ... WebApr 10, 2024 · PCR was performed in triplicate in a 20 μL mixture containing 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of each primer (5 μM), 0.4 μL of FastPfu Polymerase, and 10 ng of template DNA.
WebTransgen Ap221 02 Transstart Fastpfu Dna Polymerase, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. … WebCharacteristics. - TransStart® FastPfu Fly DNA Polymerase offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase. - PCR products can be directly cloned into …
WebTransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase. Brand TransStart® Characteristics - Extension rate is about 2-4 …
WebAll the polymerase chain reaction (PCR) processes used a 20 μL TransStart Fastpfu DNA polymerase reaction system, which included 4 μL 5× FastPfu buffer, 2 μL 2.5 mmol/L dNTPs, 0.8 μL 5... kroger worthington ohioWebJul 22, 2024 · PCR amplification was performed using the TransStart ® FastPfu Polymerase system (Transgen Biotech, Beijing, China), including 4 μL 5 × FastPfu Buffer, 2 μL dNTPs (2.5 mM), 0.8 μL each primer (5 μM), 0.4 μL FastPfu Polymerase, and 10 ng template DNA. The reaction mixture was initially denatured at 95°C for 2 min, followed by … map of majestic colonialWebDec 23, 2024 · Based on polymerase chain reaction (PCR) method and 16S rRNA sequence similarity values, ... 0.8 μL of forward and reverse primers (5 μM), 0.4 μL of FastPfu Polymerase and 10 ng of template DNA were for PCR amplification in triplicate. PCR amplicons were purified by AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, … map of mainz germanyhttp://school.freekaoyan.com/bj/wswyjs/2024/12-26/16405189191500959.shtml map of maitland florida and surrounding areaWebMar 8, 2024 · For each sample, PCR reactions for bacteria were carried out in triplicate 20-μL reactions with 0.4 μL of each primer at 5 μmol⋅L −1, 10 ng template DNA, 2 μL dNTPs … kroger worthington pharmacyWebJul 5, 2024 · PCR reactions were performed in a 20 μL mixture containing 4 μL of ×5 FastPfu Buffer, 2 μL of 2.5 mm dNTPs, 0.8 μL of each primer (5 μM), 0.4 μL of FastPfu Polymerase, and 10 ng of template DNA. ... (5 μM), 0.4 μL of FastPfu Polymerase, and 10 ng of template DNA. EmPCR products were prepared using the Roche emPCRAmp … map of majora\u0027s maskWebApr 11, 2024 · PCR reactions were performed in quadruplicate using a 20 μL mixture consisting of 4 μL of 5× TransStart FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of Forward Primer and Reverse Primer (5 μM), 0.4 μL of FastPfu Polymerase, 0.2 μL BSA, 10 ng Template DNA, and supplemented with sterile water to 20 μL. The qPCR product was … map of major airports