WebFeb 11, 2011 · To understand how SUMO modification regulates PCNA, we generated a split SUMO-modified PCNA protein and showed that it supports cell viability and stimulates DNA polymerase δ activity. We then determined its X-ray crystal structure and found that SUMO occupies a position on the back face of the PCNA ring, which is distinct from the … WebAug 20, 2024 · The crystal structures of the PCNA in complex with DNMT1 PIP box peptide have been deposited in the Protein Data Bank under accession code 6K3A. Conflict of interest statement. None declared. Acknowledgements. We would like to thank the beamline staff at the Photon Factory for X-ray data collection. We also thank Dr. A. …

RCSB PDB - 6T7X: Crystal structure of PCNA from P. abyssi

WebSep 5, 2003 · The structural basis of the FEN1-PCNA interaction was revealed by the crystal structure of the complex between human FEN1 and PCNA. The main interface involves the C-terminal tail of FEN1, which forms two beta-strands connected by a short helix, the betaA-alphaA-betaB motif, participating in beta-beta and hydrophobic … WebMay 27, 2016 · The structure of DVC PIP -PCNA complex was resolved at 2.95 Å. Data collection and refinement statistics were presented in Table 2. The complex crystal belongs to space group P4 3 2 12, containing three protomers in an asymmetric unit, with one PIP peptide bound to each protomer. fishing for tilapia in florida

RCSB PDB - 3VKX: Structure of PCNA

WebMar 28, 2024 · Mapping the most perturbed residues onto the structure of the PCNA-FBH1 PIP complex delineated the FBH1 PIP-binding pocket (Figure 2F). Substantial overlap of … WebMay 4, 2014 · In the same year, another group published a method to produce mono-ubiquitinated PCNA by splitting the protein into two polypeptides that self-assemble by non-covalent interactions. The crystal structure of split mono-ubiquitinated PCNA was solved to a resolution of 2.8 Å and deposited in the Protein Data Bank (PDB codes 3L10 and 3L0W). WebAug 20, 2024 · Here, we report the crystal structure of PCNA bound to DNMT1 PIP box peptide. The structure reveals the detailed interaction between PCNA and DNMT1 PIP box; conserved glutamine and hydrophobic/aromatic residues in the PIP box are recognized by the Q- and hydrophobic pockets of PCNA, respectively. The structure also shows novel … canberra to thredbo by car